Basic Blue 9 also known as methylthioninium chloride, is a medication and dye. As a medication, it is mainly used to treat methemoglobinemia.Specifically, it is used to treat methemoglobin levels that are greater than 30% or in which there are symptoms despite oxygen therapy.[3] It has previously been used for cyanide poisoning and urinary tract infections, but this use is no longer recommended. It is typically given by injection into a vein.

Chemical Name: Methylene blue

CAS No.: 61-73-4

Molecular Formula: C16H18ClN3S

Molecular weight: 319.85

Appearance: Dark green uniform powder

Strength: 107%

Methylene blue is employed as a medication for the treatment of methemoglobinemia. This can arise from ingestion of certain pharmaceuticals, toxins, or broad beans. Normally, through the NADH or NADPH dependent methemoglobin reductaseenzymes, methemoglobin is reduced back to hemoglobin. When large amounts of methemoglobin occur secondary to toxins, methemoglobin reductases are overwhelmed.

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Methylene blue is a heterocyclic aromatic chemical compound with the chemical formula C16H18N3SCl. It has many uses in biology and chemistry; for example, it can be used as a stain and as a pharmaceutical drug. At room temperature it appears as a solid, odorless, dark green powder, that yields a blue solution when dissolved in water. The hydrated form has 3 molecules of water per unit of methylene blue. Methylene blue should not be confused with methyl blue, another histology stain, new methylene blue, nor with the methyl violets often used as pH indicators.

1. Mainly used for dyeing cotton, acrylic, silk, paper, also used for the coloration of bamboo and wood, used to make ink, lake, and can also be used for dyeing biological bacteria.
   2. Used as redox indicator, adsorption indicator and biological dye
   3. Bacterial stains. The serum protein biochemical test is accompanied by a mixed indicator. Prepare exfoliated cells to test staining solution. Preparation of alkaline methylene blue staining solution. Redox indicator.
   4. Determination of tin. A titrant is reduced to precipitate perchlorate and citrate. Photometric determination and molybdenum with others.