4-hydroxyethylpiperazin e ethanesulfonic acid (HEPES) is an important hydrogen ion buffering agent with good buffering capacity in the pH range of 6.8-8.2, which can control a constant pH for a long time. A concentration of 10-50mmol/L and a general culture medium containing 20mmol/L 4-hydroxyethylpiperazin e ethanesulfonic acid can provide good buffering capacity and have no toxic effect on cells.
1.Used as a biochemical reagent.
2.Biological buffering agents; Reaction buffer, pre hybridization buffer, and hybridization buffer for separating and analyzing RNA nuclear components; Used for RNA and T4RNA.
3.Molecular biology level is used to perform RNA3 '- end labeling using T4RNA ligase in Chemicalbook, and to isolate and analyze the reaction buffer components, pre hybridization buffer, and mild hybridization buffer components of nuclear RNA.
4.Cell cell adhesion, short-term cell aggregation culture, buffer solution used for cleaning tissues and cells.
HEPES (4-hydroxyethylpiperazin e ethanesulfonic acid) is a non-ionic zwitterionic buffer with high polarity, which is inert to various chemical reagents and enzymes, does not participate in or interfere with the process of biochemical reactions, and has no inhibitory effect on enzyme chemical reactions. Therefore, it can be specifically used for research on organelles and highly denatured, pH sensitive proteins and enzymes.
He maha nga wheketere kounga teitei me te mahi tahi, ka taea e koe te whakarato i nga hua o te kounga teitei me nga utu whakataetae. Ka taea hoki e matou te tuku utu mo nga hokonga nui.A ka mahi tahi matou me te maha o nga kamupene kawe utanga ngaio, ka taea te tuku hua ma te humarie me te pai ki o ringaringa. Ko te wa tuku mo nga ra 3-20 i muri i te whakapumautanga o te utu.
Items |
Paerewa |
Hua |
Te ahua | White crystalline powder | Ka rite |
Te whakamatautau | ≥99.50 | 99.98 |
Solubility(1M aq) | Clear,colorless solution | Ka rite |
PH(1% aq.) | 4.80-5.60 | 5.25 |
Heavy metals,ppm | ≤10.0 | Ka rite |
Loss on drying,% | ≤1.00 | 0.11 |
Ultraviolet absorbance 260nm(1M aq) | ≤0.100 | 0.020 |
Ultraviolet absorbance 280nm(1M aq) | ≤0.070 | 0.013 |
Fe3+ ,ppm | ≤5 | Ka rite |
SO42- ,% | ≤0.05 | Ka rite |
Whakamutunga | Qualified |
1. He wheketere koe, he kamupene hokohoko ranei?
He kamupene matou e whakauru ana i te ahumahi me te hokohoko, e whakarato ana i te ratonga kotahi-mutu. Ka taea e OEM te whakaae.
2. Kei te whakarato koe i nga tauira? He kore utu, he taapiri ranei?
Ko nga tauira kore utu.Ko te utu utauta a te tauira me utu e to taha.
3. Kei a koe etahi tiwhikete e pa ana ki te mana kounga?
ISO 9001: Tiwhikete 2008 hei whakarite i te kounga.
4. He aha te mea me whakarato e au ki te tiki korero?
Pls whakamohio mai ki a matou mo te momo hua e hiahiatia ana e koe, te rahinga ota, te wahitau me nga whakaritenga motuhake.Ka mahia te korero mo to tohutoro i te waa.
5. He aha te ahua o te tikanga utu e pai ana koe? He aha nga momo tikanga e whakaaetia ana?
Nga Tikanga Tukunga Whakaaetia: FOB,CFR,CIF,EXW;
Moni Utu Whakaaetia:USD;
Momo Utu Whakaae: T / T, Western Union; Paypal, Tauhokohoko Tauhokohoko.
Te Reo Korero:Maori.
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