4-hydroxyethylpiperazin e ethanesulfonic acid (HEPES) is an important hydrogen ion buffering agent with good buffering capacity in the pH range of 6.8-8.2, which can control a constant pH for a long time. A concentration of 10-50mmol/L and a general culture medium containing 20mmol/L 4-hydroxyethylpiperazin e ethanesulfonic acid can provide good buffering capacity and have no toxic effect on cells.

1.Used as a biochemical reagent.
2.Biological buffering agents; Reaction buffer, pre hybridization buffer, and hybridization buffer for separating and analyzing RNA nuclear components; Used for RNA and T4RNA.
3.Molecular biology level is used to perform RNA3 '- end labeling using T4RNA ligase in Chemicalbook, and to isolate and analyze the reaction buffer components, pre hybridization buffer, and mild hybridization buffer components of nuclear RNA.
4.Cell cell adhesion, short-term cell aggregation culture, buffer solution used for cleaning tissues and cells.

HEPES (4-hydroxyethylpiperazin e ethanesulfonic acid) is a non-ionic zwitterionic buffer with high polarity, which is inert to various chemical reagents and enzymes, does not participate in or interfere with the process of biochemical reactions, and has no inhibitory effect on enzyme chemical reactions. Therefore, it can be specifically used for research on organelles and highly denatured, pH sensitive proteins and enzymes.

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