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Sodium Deoxycholate
Sodium deoxycholate is a bile acid formed bybacterial action from cholate. It is usually conjugatedwith glycine or taurine. Deoxycholic acid acts as adetergent to solubilize fats for intestinal absorption, isreabsorbed itself, and is used as a choleretic anddetergent.
1.Historically sodium deoxycholate was used as an intermediate for the production of corticosteroids, which have anti-inflammatory indications.
2. It is used in the preparation and formulation of certain microbiological diagnostic media.
An emerging use of sodium deoxycholate acid is as a biological detergent to lyse cells and solubilise cellular and membrane components.
• Popular anionic, bile-acid detergent for many laboratory uses
• Effective in disrupting and dissociating many types of protein interaction
• Can be removed from solution by dialysis
• Useful for elution or regeneration of certain kinds of affinity columns
• High-purity compound with low UV absorptivity
Tine mafekitori akawanda emhando yepamusoro nekubatana kwakadzama, ayo anogona kukupa zvigadzirwa zvemhando yepamusoro nemitengo yemakwikwi. Uye isu tinogonawo kupa zvidzikiso pakutenga kwakawanda. Uye isu tinobatirana nemakambani mazhinji ehunyanzvi ekutakura zvinhu, anogona kuendesa zvigadzirwa zvakachengeteka uye zvakanaka kumaoko ako. Nguva yekutumira inenge 3-20 mazuva mushure mekusimbiswa kwekubhadhara.
| Item | Zvinotsanangurwa | Results |
| Chitarisiko | White powder, no odor and smell | Upfu chena |
| Content (The uv method to detect) | ≥ 98.0 % | 98.52 % |
| Solubility(Solvent with water and alcohol) | Clear and no deposit | Zvinoenderana |
| Arsenic salt | ≤0.0002% | Zvinoenderana |
| Than the curl | 40°~ 45° | 43.8° |
| Sodium | 5.0%~6.1% | 5.6% |
| Kurasika paKuomesa | 5.0% | 1.93% |
| Heavy Metal | ≤20ppm | Zvinoenderana |
In nature, sodium deoxycholate, which is referred to as a "secondary bile acid", is produced in the intestine from the salts of glycocholic and taurocholic acid (see cholic acid ) by the action of bacterial enzymes. Less than half of the sodium deoxycholate is absorbed by the intestine and is returned the liver where it is conjugated and released into the gall bladder.
Sodium deoxycholate is extracted from cattle bile(also known as "bovine bile" or "ox bile") , which is a by-product of the meat processing industry, in a high temperature alkaline process.
(1) Historically sodium deoxycholate was used as an intermediate for the production of corticosteroids, which have anti-inflammatory indications.
(2) It is used in the preparation and formulation of certain microbiological diagnostic media.
(3) An emerging use of sodium deoxycholate acid is as a biological detergent to lyse cells and solubilise cellular and membrane components.
(4) Popular anionic, bile-acid detergent for many laboratory uses
(5) Effective in disrupting and dissociating many types of protein interaction
(6) Useful for elution or regeneration of certain kinds of affinity columns
(7) It is most frequently used as a component of cell lysis buffers (e.g., RIPA buffer), but also has been used for liposome preparation, isolation of membrane proteins and lipids, preventing nonspecific binding in affinity chromatography and a cell culture media supplement.
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